Catalog number: A34899
The ReproSeq PGS Kit for the Ion S5 System provides all the reagents and materials necessary for a rapid, cost-effective, and comprehensive next-generation sequencing (NGS) workflow for the detection of aneuploidies across all 24 chromosomes. Using as little as 6 pg of DNA from a single cell, or input from multiple cells, the ReproSeq PGS Kit for the Ion S5 System enables accurate detection of whole chromosome and chromosome arm copy number events (down to 17 Mbp copy number events*) in as little as 10 hours**.
The ReproSeq PGS Kit with Ion 510 Chips (16 samples/run) includes all reagents for processing a total of 64 samples through a workflow that includes whole genome amplification, library construction, template preparation and chip loading on the Ion Chef instrument, and sequencing using an Ion S5 System. When combined with analysis using Ion Reporter v5.4 or later, data interpretation is enhanced with new functionality such as mosaicism detection, gender masking, and improved data plotting.
The kit contains:
Ion SingleSeq Kit (Cat. No. A34763)—includes reagents to extract, amplify, and barcode genomic DNA for preparing libraries from pre-implantation embryo biopsy samples, a process that can be completed in less than 4 hours (includes hands-on time)
Ion S5 ExT Chef Supplies, Ion S5 Chef Solutions, and Ion S5 ExT Chef PGS Reagents—contains pre-packaged single-use cartridges for automated template preparation and chip loading (5 hours, includes hands-on time) whereby library DNA is clonally amplified onto a bead surface through an emulsion-based isothermal amplification reaction
Ion S5 ExT Sequencing Solutions, Ion S5 ExT Sequencing Reagents—simplifies sequencing with ready-to-use, disposable reagent cartridges for fast and simple instrument set-up, with less than 15 minutes hands-on time, for a sequencing chemistry supporting advanced data quality and accuracy
Ion 510 Chip Kit (Cat. No. A34288)—contains 4 barcoded chips per kit for sample tracking and sequencing runs using an Ion S5 System