Catalog number: A39072
The Oncomine TCR Beta-SR Assays are robust, targeted next-generation sequencing (NGS) assays designed to accurately identify and measure the clonal expansion of T lymphocytes in formalin-fixed paraffin-embedded (FFPE) tumor samples. These assays identify unique T-cell clones through targeting of the diverse complementarity-determining region 3 (CDR3) of the T-cell receptor (TCR) gene locus in genomic DNA or RNA. The nucleotide sequence of the CDR3 region is unique to each T-cell clone and codes for the part of the TCR beta chain that is involved in antigen recognition.

Benefits of the Oncomine TCR Beta-SR Assay – DNA include:
Compatibility with a vast array of research sample types, including FFPE tissue, fresh-frozen (FF) tissue, whole blood, PBLs, and PBMCs
No need for DNA extraction from the FFPE samples with the use of Ion AmpliSeq Direct FFPE DNA Kit (Cat. No. A31136)
High sensitivity and low limit of detection (LoD) for rare clone identification through dual-barcode indexing
Efficient workflow with 48-hr sample-to-results time
Flexible input requirements ranging from 100 ng to 1 µg
Streamlined and user-friendly informatics solution with multi-sample analysis functionality