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Ion Total RNA-Seq Kit v2

Catalog number: 4475936
The Ion Total RNA-Seq Kit v2 includes the reagents needed to prepare representative cDNA libraries for strand-specific RNA sequencing on the Ion GeneStudio™ S5, Ion Proton™, or the Ion Personal Genome Machine™ (PGM™) systems. The Ion GeneStudio S5 and Ion Proton systems are ideally suited for sequencing the whole transcriptome (ribosomal RNA depleted or polyA), while the Ion GeneStudio S5 and Ion PGM systems are suitable for small RNA, as well as viral and bacterial transcriptomes. Either small RNA (such as microRNA) or whole transcriptome RNA samples can be prepared for next-generation sequencing using the Ion Total RNA-Seq Kit v2.

Additional Features of the New Ion Total RNA-Seq Kit v2:
Greater accuracy—SuperScript® VILO™ and Platinum® PCR SuperMix High Fidelity added for highest template fidelity
Barcode compatible—works with Ion Xpress™ RNA-Seq Barcode 01-16 Kit for multiplexing
Automation friendly—magnetic bead-based purification simplifies automation of library construction

Designed as a complete solution with a common workflow, the Ion Total RNA-Seq Kit v2 combines optimized reagents and protocols for discovery of small RNAs and isoforms, coding RNA, noncoding RNA, and alternative splice variants.

The Ion Total RNA-Seq Kit v2 is designed to create RNA libraries from up to 48 samples for small RNA or whole transcriptome sequencing on the Ion Proton™ or PGM™ systems.