AgriSeq – Fast and Affordable Genotyping by Sequencing Solution for Marker Assisted Selection and Breeding Programs

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Agrigenomics
AgriSeq™ – Fast and Affordable Genotyping by Sequencing Solution for Marker Assisted Selection and Breeding Programs
Abstract

The AgriSeq targeted GBS solution utilizes a highly efficient multiplexed PCR chemistry where hundreds to thousands of markers can be targeted and uniformly amplified in a single reaction. The amplicon libraries can then be barcoded and pooled for simultaneous sequencing of hundreds of samples on the Ion Torrent suite of next-generation sequencing (NGS) instruments. One of the powers of this technology is its capability to support multiple types of markers including single nucleotide polymorphisms (SNPs), multiple nucleotide polymorphisms (MNPs), insertions and deletions (InDels), and other structural variants (e.g., inversions, duplications). At a cost of pennies or less per genotype, AgriSeq targeted GBS is capable of generating up to 2.6 million genotypes per day from high-quality NGS data. Whether you’re looking to achieve more accurate marker-assisted breeding selection, or to improve your livestock parentage discrimination and trait monitoring, our solutions can help advance your plant and animal breeding programs.

The talk will detail how AgriSeq™ targeted GBS is successfully being used as a high throughput, customizable and cost-effective genotyping solution in aquaculture, animal and plant breeding studies, parentage testing and genetic purity.

Speaker
Srinivas Chadaram

Dr. Srinivas Chadaram, is a Sr. Product Manager, Agrigenomics, in the AgriBusiness Vertical at Thermo Fisher Scientific. In this role, he is responsible for the portfolio of products supporting Agrigenomics vertical – including AgriSeq GBS, Microarrays, qPCR and CE platforms supporting Agrigenomics solutions. Srinivas is based in Austin, Texas, USA. He and his team is responsible for developing T-GBS solutions for marker assisted selection & breeding application for Plant, Animal & Aquaculture breeders.

 

Srinivas is a graduate of Duke University, Fuqua School of Business with a MBA Degree, PhD in Zoology and Neurobiology from the University of Idaho and DVM from Acharya NG Ranga Agricultural University, College of Veterinary Science, Hyderabad, India. He has published several peer reviewed scientific papers.

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